Josie (Rachael Leigh Cook), Melody (Tara Reid) and Val (Rosario Dawson) are three small-town musicians with big dreams but little future. Then fate gives the Pussycats the chance of a lifetime when band manager Wyatt (Alan Cumming) of MegaRecords signs them overnight to an awesome recording contract. Suddenly, Josie and the Pussycats are living life in the fast lane with sold-out concerts, a number one single and global stardom. But it's not all limousines and private jets. The Pussycats soon discover they're being played like pawns in an evil plot by the record label's maniacal CEO Fiona (Parker Posey) to control the youth of America.