Searching for the Origin of the Butterfly Wing in a Caterpillar

• 1

• By Martin Lab

Description

The larva were removed from the water and covered with phosphate-buffered saline (PBS) in a dissecting tray. A small incision was made along the midline of either the second (forewing) or third (hindwing) thoracic segment. Using tweezers, the wing disc was partially exteriorized, being sure not to damage the connecting tissue or trachea of the wing disc