CRISPR Injection in Butterfly Eggs

• 4

• By Martin Lab

Description

Injecting Cas9/sgRNA duplexes into 1–6 h butterfly embryos at a syncytial stage (n = 5,794 eggs). As only a fraction of the dividing nuclei are edited, the resulting mosaicism can bypass the deleterious effects of developmental mutations and yields G0 escapers that survive until the adult stage for phenotypic analysis. We performed CRISPR injections in seven nymphalid species to induce frameshift mutations