Apoptosis (Programmed Cell Death) - Live and in 3D

• 1

• By Nanolive

Description

During the Real-time monitoring, we can observe the typical morphological cellular changes as blebbing, cell shrinkage and chromatin condensation.

Sample:

Apoptosis of T685A human melanoma cancer cell.

Protocol:

The cells were grown to 60% confluency in complete RPMI medium  in ibidi 35mm glass bottom dish. The time-lapse imaging experiment was conducted with a ibidi-nanolive top stage incubator tor set to 37°C and 5% CO2 for 8h, capturing images every 30 seconds.

The movie was taken using Nanolive's technology: 3D Cell Explorer. A label-free holo-tomographic microscope able to reconstruct in 3D living cells based on their optical density.