During the Real-time monitoring, we can observe the typical morphological cellular changes as blebbing, cell shrinkage and chromatin condensation.
Apoptosis of T685A human melanoma cancer cell.
The cells were grown to 60% confluency in complete RPMI medium in ibidi 35mm glass bottom dish. The time-lapse imaging experiment was conducted with a ibidi-nanolive top stage incubator tor set to 37°C and 5% CO2 for 8h, capturing images every 30 seconds.
The movie was taken using Nanolive's technology: 3D Cell Explorer. A label-free holo-tomographic microscope able to reconstruct in 3D living cells based on their optical density.